Deciphering ryanodine receptor array operation in cardiac myocytes
نویسندگان
چکیده
منابع مشابه
Deciphering ryanodine receptor array operation in cardiac myocytes
Elemental calcium signals from RYR arrays operating in cardiac myocytes have been extensively characterized with ever-improving optical methods and other innovative techniques. However, the exact nature of elemental calcium signals in terms of RYR gating in situ remains an enigma. Here, we synthesize insights gleaned from recent developments in single-channel resolution of cardiac RYR organizat...
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The clustering of ryanodine receptors (RyR2) into functional Ca2+ release units is central to current models for cardiac excitation-contraction (E-C) coupling. Using immunolabeling and confocal microscopy, we have analyzed the distribution of RyR2 clusters in rat and ventricular atrial myocytes. The resolution of the three-dimensional structure was improved by a novel transverse sectioning meth...
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Intracellular Ca(2+) release in many types of cells is mediated by ryanodine receptor Ca(2+) release channels (RyRCs) that are assembled into two-dimensional paracrystalline arrays in the endoplasmic/sarcoplasmic reticulum. However, the in situ operating mechanism of the RyRC array is unknown. Here, we found that the elementary Ca(2+) release events, Ca(2+) sparks from individual RyRC arrays in...
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The role of cardiac ryanodine receptor (RyR) gating in the initiation and propagation of calcium waves was investigated using a mathematical model comprising a stochastic description of RyR gating and a deterministic description of calcium diffusion and sequestration. We used a one-dimensional array of equidistantly spaced RyR clusters, representing the confocal scanning line, to simulate the f...
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The ryanodine receptor type 2 (RyR-2) functions as a Ca2+-induced Ca2+ release (CICR) channel on intracellular Ca2+ stores and is distributed in most excitable cells with the exception of skeletal muscle cells. RyR-2 is abundantly expressed in cardiac muscle cells and is thought to mediate Ca2+ release triggered by Ca2+ influx through the voltage-gated Ca2+ channel to constitute the cardiac typ...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 2010
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.201010416